Data/hora: 02/06/2024 18:26:19

Provedor de dados: 	Scientia Agricola	
País: 	Brazil	
Título: 	In vitro conservation of diploid banana accessions	
Autores: 	Oliveira,Roberto Pedroso de; Silva,Sebastião de Oliveira e; Silva,Keuder Magalhães da; Silveira,Daniela Garcia.	
Data: 	2000-06-01	
Ano: 	2000	
Palavras-chave: 	Musa spp.; Germplasm; In vitro conservation.	
Resumo: 	A protocol for the in vitro conservation of diploid banana accessions based on lower temperature for culture environment was developed. Twenty four genotypes, four clones per genotype were studied. After disinfection, shoot tips (0.5 cm diameter x 0.5 cm height) were established in vitro and submitted to multiplication induced by benzylaminopurine. Twelve explants (0.6 cm diameter x 1.2 cm height) from each accession were transferred to the MS medium supplemented with 20 g L-1 sucrose and 2 g L-1 Phytagel, without growth regulators, under light intensity of 2000 lux, 16-hour photoperiod, at temperatures of: 17 ± 2ºC, 22 ± 2ºC and 26 ± 2ºC. The development of plantlets was evaluated during 450 days and the survived plantlets were acclimated under greenhouse conditions. There were no differences among genotypes concerning the behavior during in vitro conservation; all genotypes showed potential to be conserved for a long time. The interval for transference should be 180, 360 and 450 days under the mean temperatures of 26, 22 and 17ºC, respectively.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000200008	
Editor: 	São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"	
Relação: 	10.1590/S0103-90162000000200008	
Formato: 	text/html	
Fonte: 	Scientia Agricola  v.57 n.2 2000	
Fonte: 	info:eu-repo/semantics/openAccess